刘东 教授

南通大学生命科学学院，教授，博士生导师

教育部神经再生重点实验室，课题组长

南通市发育与人类相关疾病重点实验室，主任

出生年月：1980年8月

E-mail: liudongtom@gmail.com；tom@ntu.edu.cn

网页：https://smkxxy.ntu.edu.cn/ldsys/main.htm

一、研究方向与简介：

1、基因编辑与疾病模型的构建

2、器官特异性血管血管内皮细胞的遗传调控

3、基于血管内皮细胞和神经元的化合物筛选

4、听、平衡觉的发育与遗传调控

二、教育:

2011年9月：德国马克思德尔布吕克分子医学中心、柏林自由大学，分子医学专业博士

三、工作经历:

2019年3月-至今：南通大学神经再生重点实验室，生命科学院，教授

2017年11月-至今：南通大学神经再生重点实验室，教授

2012年3月-2017年11月：南通大学神经再生重点实验室，副教授

四、主持课题:

2024.01-2026.12: 国家自然科学基金-重大研究计划

项目批准号：92368104；项目名称：基于高分辨活体成像与单细胞组学解析内耳毛细胞自发再生的机制

2024.01-2025.12: 国家自然科学基金-原创探索计划项目

项目批准号：32350017；项目名称：一种新型细胞器Cuticulosome”的组成、结构及其参与磁感应功能的生物学机制研究

2019.09-2024.08: 国家重点研发计划子课题

项目批准号：2018YFA0801004；项目名称：心血管发育与再生突变体系统研制

2019.01-2022.12: 国家自然科学基金-面上项目

项目批准号：81870359；项目名称：Aquaporins调控血管新生过程中管腔形成的机制研

2016.01-2019.12: 国家自然科学基金-面上项目

项目批准号：81570447；项目名称：旁分泌因子Maeg调控血管新生的机制研究

2013.01-2015.12: 国家自然科学基金-青年项目

项目批准号：31201083；项目名称：miR-146a和miR-10a/b在斑马鱼胚胎血管发育中的功能研究

2018.07-2021.06: 江苏省自然科学基金-杰出青年基金项目

项目批准号：BK20180048；项目名称：利用斑马鱼模型鉴定全新的血管新生调控因子

2012.07-2015.06: 江苏省自然科学基金-青年基金项目

项目批准号：BK2012228；项目名称：MicroRNA(miR-22a/20a/17a)在斑马鱼动静脉分化及胚胎血 管发育中的功能研究

2017.07-2020.06: 江苏省高校自然科学研究项目-重大项目

项目批准号：17KJA180008；项目名称：EGF 家族基因调控神经再生过程中血 管化的机制研究

2012.07-2014.06: 江苏省高校自然科学研究项目-面上项目

项目批准号：12KJB180010；项目名称：miRNA在 斑 马 鱼 胚 胎 血 管 系 统 发 育 中 的功能研究

2019.06-2021.06: 江苏省第五期“333工程”科研资助项目

2016-2019: 江苏省“六大人才高峰”高层次人才培养对象

2014-2016: 江苏省“双创计划”博士计划，境外世界名校创新类计划

2016-2022: 与 “齐鲁制药” 合作技术开发项目4个

五、荣誉及专业学会会员: 

2018年，江苏省自然科学基金“杰出青年基金”获得者

2018年，江苏省第五期“333高层次人才培养工程”培养对象

2016年，江苏省“六大人才高峰” 高层次人才培养对象

2013年，江苏省“双创计划”双创博士境外名校创新类

2023年，江苏省青蓝工程中青年学术带头人

2022年，南通市第六期江海英才培养对象

美国心脏协会American Heart Association会员

中国解剖学会再生医学专业委员会委员

中国解剖学会青年工作委员会委员

中国生物物理学会听觉分会委员，青年委员会常务委员

中国第四届水生实验动物专业委员会委员

中国研究型医院学会运动医学专业委员会委员

江苏省生物技术学会第八届理事会常务理事

江苏省生物物理学会理事

江苏省发育与细胞学会理事

江苏省神经科学会理事

江苏省神经科学会青年委员会委员

江苏省医学会第一届听力学分会委员

江苏省发育生物学会听觉科学专业委员会副主任委员

复旦大学优秀毕业生

六、已毕业研究生：

2015年，李丽萍 

2016年，袁玮、朱晨文、柴琳琳 

2017年，秦银银 、祁嘉玲

2018年，高煜、殷振华 

2019 年，王晓宁、肖薇、史媛媛;

2020 年，钱佩佩、赵金湘

2021年，张旭、许梦婷

2022年，杨超、高亚静

2023年，尹国力、王晓宁 (博士生)

七、代表性论文：

1. Wang XN., Zhao JX., Xu JH., Li BW., Liu X., Xie GC., Duan XC., Liu, D., Noncaloric monosaccharides induce excessive sprouting angiogenesis in zebrafish via foxo1a-marcksl1a signal. Elife 2024. (Accepted，通讯作者)

2. Chen C.; Qin Y.; Xu Y.; Wang X.; Lei W.; Shen X.; Chen L.; Wang L.; Gong J.; Wang Y.; Hu S.;  Liu, D., Aquaporins enriched in endothelial vacuole membrane regulate the diameters of microvasculature in hyperglycemia. Cardiovascular Research 2024. (Accepted，通讯作者)

3. You, B.;  Pan, S.;  Gu, M.;  Zhang, K.;  Xia, T.;  Zhang, S.;  Chen, W.;  Xie, H.;  Fan, Y.;  Yao, H.;  Cheng, T.;  Zhang, P.;  Liu, D.; You, Y., Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation. J Extracell Vesicles 2022, 11 (5), e12221. (通讯作者)

4. You, B.;  Xia, T.;  Gu, M.;  Zhang, Z.;  Zhang, Q.;  Shen, J.;  Fan, Y.;  Yao, H.;  Pan, S.;  Lu, Y.;  Cheng, T.;  Yang, Z.; He, X.;  Zhang, H.;  Shi, M.;  Liu, D.; You, Y., AMPK-mTOR-Mediated Activation of Autophagy Promotes Formation of Dormant Polyploid Giant Cancer Cells. Cancer Research 2022, 82 (5), 846-858. (通讯作者)

5. Li, N.;  Zhou, P.;  Tang, H.;  He, L.;  Fang, X.;  Zhao, J.;  Wang, X.;  Qi, Y.;  Sun, C.;  Lin, Y.;  Qin, F.;  Yang, M.; Zhang, Z.;  Liao, C.;  Zheng, S.;  Peng, X.;  Xue, T.;  Zhu, Q.;  Li, H.;  Li, Y.;  Liu, L.;  Huang, J.;  Liu, L.;  Peng, C.; Kaindl, A. M.;  Gecz, J.;  Han, D.;  Liu, D.;  Xu, K.; Hu, H., In-depth analysis reveals complex molecular aetiology in a cohort of idiopathic cerebral palsy. Brain 2022. (通讯作者)

6. Zhang, L.;  Gao, Y.;  Zhang, R.;  Sun, F.;  Cheng, C.;  Qian, F.;  Duan, X.;  Wei, G.;  Sun, C.;  Pang, X.;  Chen, P.;  Chai, R.;  Yang, T.;  Wu, H.; Liu, D., THOC1 deficiency leads to late-onset nonsyndromic hearing loss through p53-mediated hair cell apoptosis. PLoS Genets 2020, 16 (8), e1008953. (通讯作者)

7. Shi, Y.;  Duan, X.;  Xu, G.;  Wang, X.;  Wei, G.;  Dong, S.;  Xie, G.; Liu, D., A ribosomal DNA-hosted microRNA regulates zebrafish embryonic angiogenesis. Angiogenesis 2019, 22 (2), 211-221. (通讯作者)

8. Bao, L.;  You, B.;  Shi, S.;  Shan, Y.;  Zhang, Q.;  Yue, H.;  Zhang, J.;  Zhang, W.;  Shi, Y.;  Liu, Y.;  Wang, X.;  Liu, D.; You, Y., Metastasis-associated miR-23a from nasopharyngeal carcinoma-derived exosomes mediates angiogenesis by repressing a novel target gene TSGA10. Oncogene 2018, 37 (21), 2873-2889. (通讯作者)

9. Wang, F.;  Liu, D.;  Zhang, R. R.;  Yu, L. W.;  Zhao, J. Y.;  Yang, X. Y.;  Jiang, S. S.;  Ma, D.;  Qiao, B.;  Zhang, F.;  Jin, L.;  Gui, Y. H.; Wang, H. Y., A TBX5 3'UTR variant increases the risk of congenital heart disease in the Han Chinese population. Cell Discovery 2017, 3, 17026. (共同一作)

10. Wang, X.;  Ling, C. C.;  Li, L.;  Qin, Y.;  Qi, J.;  Liu, X.;  You, B.;  Shi, Y.;  Zhang, J.;  Jiang, Q.;  Xu, H.;  Sun, C.; You, Y.;  Chai, R.; Liu, D., MicroRNA-10a/10b represses a novel target gene mib1 to regulate angiogenesis. Cardiovascular Research 2016, 110 (1), 140-50. (通讯作者)

11. Qi, J.;  Dong, Z.;  Shi, Y.;  Wang, X.;  Qin, Y.;  Wang, Y.; Liu, D., NgAgo-based fabp11a gene knockdown causes eye developmental defects in zebrafish. Cell Research 2016, 26 (12), 1349-1352. (通讯作者)

八、其他SCI论文

2023年

1.   Yin G, Qian F, Yao J, Wang Z, Wang X, Liu D, et al. ftr82 is necessary for hair cell morphogenesis and auditory function during zebrafish development. Journal of genetics and genomics  2023;50(2):77-86. (通讯作者) 

2. Zhu Q, Tan M, Wang C, Chen Y, Wang C, Zhang J, et al. Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos. Cell Biosci. 2023;13(1):130. (通讯作者)

3. Yuan W, Zhang Q, Gu D, Lu C, Dixit D, Gimple RC, et al. Dual Role of CXCL8 in Maintaining the Mesenchymal State of Glioblastoma Stem Cells and M2-Like Tumor-Associated Macrophages. Clin Cancer Res. 2023;29(18):3779-92.

4. Wang X, Gu X, Wang C, He Y, Liu D, Sun S, et al. Loss of ndrg2 Function Is Involved in Notch Activation in Neuromast Hair Cell Regeneration in Zebrafish. Molecular neurobiology. 2023;60(6):3100-12. (通讯作者)

5. Wang C, Wang X, Zheng H, Yao J, Xiang Y, Liu D. The ndrg2 Gene Regulates Hair Cell Morphogenesis and Auditory Function during Zebrafish Development. Int J Mol Sci. 2023;24(12). (通讯作者)

6. Shi C, Mammadova-Bach E, Li C, Liu D, Anders HJ. Pathophysiology and targeted treatment of cholesterol crystal embolism and the related thrombotic angiopathy. FASEB J. 2023;37(10):e23179.

7. Gao X, Chen C, Shi S, Qian F, Liu D, Gong J. Tight Junctions in the Auditory System: Structure, Distribution and Function. Curr Protein Pept Sci. 2023;24(3):203-14. (通讯作者)

8. Dai YB, Gao X, Liu D, Gong J. The role of Rho GTPase family in cochlear hair cells and hearing. Neural Regen Res. 2023;18(10):2167-72. (通讯作者)

9. Cai CY, Liang HY, Zhou T, Yang C, Yin JJ, Yao MH, et al. High-intensity interval training ameliorates chronic unpredictable mild stress-induced depressive behaviors via HDAC2-BDNF signaling in the ventral hippocampus. Brain Res. 2023;1816:148480. (通讯作者)

10. Zhao S, Liu D. Applications of genetic code expansion and photosensitive UAAs in studying membrane proteins. Open Life Sci. 2023 Oct 10;18(1):20220752. (通讯作者)

2022年

1. Chen, C.; Liu, D., Establishment of Zebrafish Models for Diabetes Mellitus and Its Microvascular Complications. J Vasc Res 2022, 1-9. (通讯作者)

2. Zheng, S.;  Tang, D.;  Wang, X.;  Liu, C.;  Zuo, N.;  Yan, R.;  Wu, C.;  Ma, J.;  Wang, C.;  Xu, H.;  He, Y.;  Liu, D.; Liu, S., Kif15 Is Required in the Development of Auditory System Using Zebrafish as a Model. Frontiers in molecular neuroscience 2022, 15, 844568. (通讯作者)

3. Xu, G.;  Huang, Z.;  Sheng, J.;  Gao, X.;  Wang, X.;  Garcia, J. Q.;  Wei, G.;  Liu, D.; Gong, J., FGF binding protein 3 is required for spinal cord motor neuron development and regeneration in zebrafish. Exp Neurol 2022, 348, 113944. (通讯作者)

4. Wei, G.;  Zhang, X.;  Cai, C.;  Sheng, J.;  Xu, M.;  Wang, C.;  Gu, Q.;  Guo, C.;  Chen, F.;  Liu, D.; Qian, F., Dual-Specificity Phosphatase 14 Regulates Zebrafish Hair Cell Formation Through Activation of p38 Signaling Pathway. Front Cell Neurosci 2022, 16, 840143. (通讯作者)

5. Sheng, J.;  Xu, J.;  Geng, K.; Liu, D., Sema6D Regulates Zebrafish Vascular Patterning and Motor Neuronal Axon Growth in Spinal Cord. Frontiers in molecular neuroscience 2022, 15, 854556. (通讯作者)

6. Sheng, J.;  Gong, J.;  Shi, Y.;  Wang, X.; Liu, D., MicroRNA-22 coordinates vascular and motor neuronal pathfinding via sema4 during zebrafish development. Open Biol 2022, 12 (4), 210315. (通讯作者)

7. Qian, P.;  Kang, J.;  Liu, D.; Xie, G., Single Cell Transcriptome Sequencing of Zebrafish Testis Revealed Novel Spermatogenesis Marker Genes and Stronger Leydig-Germ Cell Paracrine Interactions. Front Genet 2022, 13, 851719. (通讯作者)

8. Qian, F.;  Jiang, X.;  Chai, R.; Liu, D., The Roles of Solute Carriers in Auditory Function. Front Genet 2022, 13, 823049. (通讯作者)

9. Lv, F.;  Ge, X.;  Qian, P.;  Lu, X.;  Liu, D.; Chen, C., Neuron navigator 3 (NAV3) is required for heart development in zebrafish. Fish physiology and biochemistry 2022. (通讯作者)

10. Hu, S. Q.;  Xu, H. M.;  Qian, F. P.;  Chen, C. S.;  Wang, X.;  Liu, D.; Cheng, L., Interferon regulatory factor-7 is required for hair cell development during zebrafish embryogenesis. Dev Neurobiol 2022, 82 (1), 88-97. (通讯作者)

11. Zheng, Y. Q.;  Suo, G. H.;  Liu, D.;  Li, H. Y.;  Wu, Y. J.; Ni, H., Nexmifa Regulates Axon Morphogenesis in Motor Neurons in Zebrafish. Frontiers in molecular neuroscience 2022, 15, 848257. (通讯作者)

12. Cui C, Zhang L, Qian F, Chen Y, Huang B, Wang F, Wang D, Lv J, Wang X, Yan Z, Guo L, Li GL, Shu Y, Liu D, Li H. A humanized murine model, demonstrating dominant progressive hearing loss caused by a novel KCNQ4 mutation (p.G228D) from a large Chinese family. Clin Genet. 2022 May 22. doi: 10.1111/cge.14164. (通讯作者)

13. Li C, Li H, Yao X, Liu D, Wang Y, Huang X, Yang Z, Tao W, Zhao JY, Wang H. Master microRNA-222 regulates cardiac microRNA maturation and triggers Tetralogy of Fallot. Signal Transduct Target Ther. 2022 May 30;7(1):165. doi: 10.1038/s41392-022-00993-1.

14. Zhao J, Wei G, Zhu J, Liu D, Qin B. Expression analysis of nel during zebrafish embryonic development. Gene Expr Patterns. 2022 Jun 9;45:119258. doi: 10.1016/j.gep.2022.119258. (通讯作者)

15. Lv F, Ge X, Qian P, Lu X, Liu D, Chen C. Neuron nvigator 3 (NAV3) is required for heart development in zebrafish. Fish Physiol Biochem. 2022 Feb;48(1):173-183. (通讯作者)

16. Xuchu Duan, Yuanyuan, Shi, Shu Zhao, Lili Yao, Jiajing Sheng, DongLiu. Foxc1a regulates zebrafish vascular integrity and brain vascular development through targeting amotl2a and ctnnb1. Microvascular Research. June 2022, 104400 (通讯作者)

17. Zhou J, Liang W, Wang J, Chen J, Liu D, Wang X, et al. An epileptic encephalopathy associated GABRG2 missense mutation leads to pre- and postsynaptic defects in zebrafish. Human molecular genetics. 2022;31(19):3216-3 

18. Zhao Y, Yang Q, Liu D, Liu T, Xing L. Neurotoxicity of nanoparticles: Insight from studies in zebrafish. Ecotoxicol Environ Saf. 2022;242:113896.

19. Yuan F, Qian PP, Wang X, Sheng JJ, Liu D, Gong J. [Establishment of a microtubule-fluorescent fusion protein mosaically labeled zebrafish motor neuron system]. [Acta physiologica Sinica]. 2022;74(3):411-8 

20. Wang X, Ge X, Qin Y, Liu D, Chen C. Ifi30 Is Required for Sprouting Angiogenesis During Caudal Vein Plexus Formation in Zebrafish. Frontiers in physiology. 2022;13:919579. (通讯作者)

21. Wang S, Qin Y, Sheng J, Duan X, Shen L, Liu D. Aquaporin 8ab is required in zebrafish embryonic intestine development. Acta Biochim Biophys Sin (Shanghai). 2022;54(7):952-60. (通讯作者)

22. Shi C, Zhao D, Lyubenov L, Motrapu M, Li N, Steiger S, et al. Neutrophil circadian rhythm is associated with different outcomes of acute kidney injury due to cholesterol crystal embolism. Front Cardiovasc Med. 2022;9:974759. 

23. Qian F, Wei G, Gao Y, Wang X, Gong J, Guo C, et al. Single-cell RNA-sequencing of zebrafish hair cells reveals novel genes potentially involved in hearing loss. Cell Mol Life Sci. 2022;79(7):385. (通讯作者)

24. Lu Y, Tang D, Zheng Z, Wang X, Zuo N, Yan R, et al. Cingulin b Is Required for Zebrafish Lateral Line Development Through Regulation of Mitogen-Activated Protein Kinase and Cellular Senescence Signaling Pathways. Frontiers in molecular neuroscience. 2022;15:844668. (通讯作者)

25. Liu JH, Yang C, Gu QX, Liang HY, Liu D, Liu J, et al. [Effect of high-intensity interval exercise on depression-related behavior in mice]. [Acta physiologica Sinica]. 2022;74(2):165-76.

26. Cui C, Wang D, Huang B, Wang F, Chen Y, Lv J, et al. Precise detection of CRISPR-Cas9 editing in hair cells in the treatment of autosomal dominant hearing loss. Mol Ther Nucleic Acids. 2022;29:400-12.

2021年

1. Zhou, J.;  Liang, W.;  Wang, J.;  Chen, J.;  Liu, D.;  Wang, X.;  Wu, Y.;  Zhang, Q.; Shen, D., An epileptic encephalopathy associated GABRG2 missense mutation leads to pre- and postsynaptic defects in zebrafish. Human molecular genetics 2021.

2. Zheng, Y.;  Zhao, J.;  Li, J.;  Guo, Z.;  Sheng, J.;  Ye, X.;  Jin, G.;  Wang, C.;  Chai, W.;  Yan, J.;  Liu, D.; Liang, X., SARS-CoV-2 spike protein causes blood coagulation and thrombosis by competitive binding to heparan sulfate. Int J Biol Macromol 2021, 193 (Pt B), 1124-1129. (通讯作者)

3. Zhang, K.;  Liu, D.;  Zhao, J.;  Shi, S.;  He, X.;  Da, P.;  You, Y.; You, B., Nuclear exosome HMGB3 secreted by nasopharyngeal carcinoma cells promotes tumour metastasis by inducing angiogenesis. Cell Death Dis 2021, 12 (6), 554. (共同一作)

4. Xie, G.;  Zhang, X.;  Lv, F.;  Sang, M.;  Hu, H.;  Wang, J.; Liu, D., Nanopore Sequencing and Hi-C Based De Novo Assembly of Trachidermus fasciatus Genome. Genes (Basel) 2021, 12 (5). (通讯作者)

5. Shi, L.;  Chen, C.;  Yin, Z.;  Wei, G.;  Xie, G.; Liu, D., Systematic profiling of early regulators during tissue regeneration using zebrafish model. Wound Repair Regen 2021, 29 (1), 189-195. (通讯作者)

6. Pang, X. H.;  Zheng, X. Y.;  Lin, Y.;  Zheng, H.;  Xu, J.;  Liu, D.;  Jin, C. Y.;  Zhang, L. P.;  Zhang, Y. T.;  Chu, J. S.;  Chai, Y. C.; Yang, T., A Novel p.Tyr129His Variant in SIX1 Leads to Dominant, Delayed-onset Hearing Loss with Possible Association with Congenital Anosmia. Biomed Environ Sci 2021, 34 (4), 314-318.

7. Li, M.;  Liu, J.;  Liu, D.;  Duan, X.;  Zhang, Q.;  Wang, D.;  Zheng, Q.;  Bai, X.; Lu, Z., Naringin attenuates cisplatin- and aminoglycoside-induced hair cell injury in the zebrafish lateral line via multiple pathways. J Cell Mol Med 2021, 25 (2), 975-989.

8. Hu, Z.;  Zhang, C.;  Wang, S.;  Gao, S.;  Wei, J.;  Li, M.;  Hou, L.;  Mao, H.;  Wei, Y.;  Qi, T.;  Liu, H.;  Liu, D.;  Lan, F.;  Lu, D.;  Wang, H.;  Li, J.; Wang, Y., Discovery and engineering of small SlugCas9 with broad targeting range and high specificity and activity. Nucleic acids research 2021, 49 (7), 4008-4019.

9. Gong, J.;  Qian, P.;  Hu, Y.;  Guo, C.;  Wei, G.;  Wang, C.;  Cai, C.;  Wang, H.; Liu, D., Claudin h Is Essential for Hair Cell Morphogenesis and Auditory Function in Zebrafish. Front Cell Dev Biol 2021, 9, 663995. (通讯作者)

2020年

1. Zhang, Z.;  Hou, H.;  Yu, S.;  Zhou, C.;  Zhang, X.;  Li, N.;  Zhang, S.;  Song, K.;  Lu, Y.;  Liu, D.;  Lu, H.; Xu, H., Inflammation-induced mammalian target of rapamycin signaling is essential for retina regeneration. Glia 2020, 68 (1), 111-127.

2. Zhang, Y.;  Wang, Y.;  Yao, X.;  Wang, C.;  Chen, F.;  Liu, D.;  Shao, M.; Xu, Z., Rbm24a Is Necessary for Hair Cell Development Through Regulating mRNA Stability in Zebrafish. Front Cell Dev Biol 2020, 8, 604026.

3. Wang, B.;  Xu, M.;  Li, M.;  Wu, F.;  Hu, S.;  Chen, X.;  Zhao, L.;  Huang, Z.;  Lan, F.;  Liu, D.; Wang, Y., miR-25 Promotes Cardiomyocyte Proliferation by Targeting FBXW7. Mol Ther Nucleic Acids 2020, 19, 1299-1308. (通讯作者)

4. Shen, D.;  Chen, J.;  Liu, D.;  Shen, M.;  Wang, X.;  Wu, Y.;  Ke, S.;  Macdonald, R. L.; Zhang, Q., The GABRG2 F343L allele causes spontaneous seizures in a novel transgenic zebrafish model that can be treated with suberanilohydroxamic acid (SAHA). Ann Transl Med 2020, 8 (23), 1560.

5. Qian, F.;  Wang, X.;  Yin, Z.;  Xie, G.;  Yuan, H.;  Liu, D.; Chai, R., The slc4a2b gene is required for hair cell development in zebrafish. Aging (Albany NY) 2020, 12 (19), 18804-18821. (通讯作者)

6. Liu, C.;  Zhao, J.;  Zhang, X.;  Wei, G.;  Hao, W.;  Wang, X.;  Yang, C.;  Shi, Y.; Liu, D., Biomass-derived cellulose nanoparticles display considerable neurotoxicity in zebrafish. Int J Biol Macromol 2020, 165 (Pt B), 1783-1792. (通讯作者)

7. Hu, Z.;  Wang, S.;  Zhang, C.;  Gao, N.;  Li, M.;  Wang, D.;  Wang, D.;  Liu, D.;  Liu, H.;  Ong, S. G.;  Wang, H.; Wang, Y., A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope. PLoS Biol 2020, 18 (3), e3000686.

8. Gong, J.;  Hu, S.;  Huang, Z.;  Hu, Y.;  Wang, X.;  Zhao, J.;  Qian, P.;  Wang, C.;  Sheng, J.;  Lu, X.;  Wei, G.; Liu, D., The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish. Frontiers in molecular neuroscience 2020, 13, 34. (通讯作者)

9. Gao, C.;  Guo, C.;  Peng, Q.;  Cao, J.;  Shohat-Ophir, G.;  Liu, D.; Pan, Y., Sex and Death: Identification of Feedback Neuromodulation Balancing Reproduction and Survival. Neuroscience bulletin 2020, 36 (12), 1429-1440.

2019年

1. Zhang, H.;  Gao, Y.;  Qian, P.;  Dong, Z.;  Hao, W.;  Liu, D.; Duan, X., Expression analysis of Rab11 during zebrafish embryonic development. BMC Dev Biol 2019, 19 (1), 25.

2. Yu, B.;  Yao, C.;  Wang, Y.;  Mao, S.;  Wang, Y.;  Wu, R.;  Feng, W.;  Chen, Y.;  Yang, J.;  Xue, C.;  Liu, D.; Ding, F.; Gu, X., The Landscape of Gene Expression and Molecular Regulation Following Spinal Cord Hemisection in Rats. Frontiers in molecular neuroscience 2019, 12, 287.

3. Wang, Y. M.;  Zheng, Y. F.;  Yang, S. Y.;  Yang, Z. M.;  Zhang, L. N.;  He, Y. Q.;  Gong, X. H.;  Liu, D.;  Finnell, R. H.;  Qiu, Z. L.;  Du, Y. S.; Wang, H. Y., MicroRNA-197 controls ADAM10 expression to mediate MeCP2's role in the differentiation of neuronal progenitors. Cell Death Differ 2019, 26 (10), 1863-1879.

4. Wang, D.;  Zhang, C.;  Wang, B.;  Li, B.;  Wang, Q.;  Liu, D.;  Wang, H.;  Zhou, Y.;  Shi, L.;  Lan, F.; Wang, Y., Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning. Nat Commun 2019, 10 (1), 4284.

5. Shi, Y.;  Wang, X.;  Shi, Y.;  Zhang, J.;  Zhao, S.;  Yin, Z.;  Xu, G.;  Duan, X.;  Guo, Z.;  Liang, X.; Liu, D., Two Compounds Isolated From Ganglioside GM1 Promote Angiogenesis in Zebrafish. J Cardiovasc Pharmacol 2019, 74 (1), 71-79. (通讯作者)

6. Qin, Y.;  Wang, S.;  Duan, X.; Liu, D., Expression analysis of the aquaporins during zebrafish embryonic development. Gene expression patterns : GEP 2019, 32, 38-43. (通讯作者)

7. Li, L.;  Zhu, M.;  Wu, W.;  Qin, B.;  Gu, J.;  Tu, Y.;  Chen, J.;  Liu, D.;  Shi, Y.;  Liu, X.;  Sang, A.; Ding, D., Brivanib, a multitargeted small-molecule tyrosine kinase inhibitor, suppresses laser-induced CNV in a mouse model of neovascular AMD. J Cell Physiol 2019.

8. Dong, Z.;  Wu, S.;  Zhu, C.;  Wang, X.;  Li, Y.;  Chen, X.;  Liu, D.;  Qiang, L.;  Baas, P. W.; Liu, M., Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-mediated kif15 mutations accelerate axonal outgrowth during neuronal development and regeneration in zebrafish. Traffic 2019, 20 (1), 71-81.

9. Zhang, K.;  Zhang, H.;  Zhou, H.;  Crookes, D.;  Li, L.;  Shao, Y.; Liu, D., Zebrafish Embryo Vessel Segmentation Using a Novel Dual ResUNet Model. Comput Intell Neurosci 2019, 2019, 8214975. (通讯作者)

2018年

1. Yan, W.;  Liu, W.;  Qi, J.;  Fang, Q.;  Fan, Z.;  Sun, G.;  Han, Y.;  Zhang, D.;  Xu, L.;  Wang, M.;  Li, J.;  Chen, F.;  Liu, D.;  Chai, R.; Wang, H., A Three-Dimensional Culture System with Matrigel Promotes Purified Spiral Ganglion Neuron Survival and Function In Vitro. Molecular neurobiology 2018, 55 (3), 2070-2084. (通讯作者)

2. Shan, Y.;  You, B.;  Shi, S.;  Shi, W.;  Zhang, Z.;  Zhang, Q.;  Gu, M.;  Chen, J.;  Bao, L.;  Liu, D.; You, Y., Hypoxia-Induced Matrix Metalloproteinase-13 Expression in Exosomes from Nasopharyngeal Carcinoma Enhances Metastases. Cell Death Dis 2018, 9 (3), 382. (通讯作者)

3. Li, B.;  Yu, L.;  Liu, D.;  Yang, X.;  Zheng, Y.;  Gui, Y.; Wang, H., MIB1 Mutations Reduce Notch Signaling Activation and Contribute to Congenital Heart Disease. Clin Sci (Lond) 2018.

4. Gong, J.;  Chai, L.;  Xu, G.;  Ni, Y.; Liu, D., The expression of natriuretic peptide receptors in developing zebrafish embryos. Gene expression patterns : GEP 2018, 29, 65-71. (通讯作者)

2017年

1. Zhang, J.;  Qi, J.;  Wu, S.;  Peng, L.;  Shi, Y.;  Yang, J.;  Yin, Z.;  Gao, Y.;  Wang, C.;  Gong, J.;  Zhang, H.;  Zhang, J.; Liu, D., Fatty Acid Binding Protein 11a Is Required for Brain Vessel Integrity in Zebrafish. Frontiers in physiology 2017, 8, 214. (通讯作者)

2. Xie, Y.;  Wang, D.;  Lan, F.;  Wei, G.;  Ni, T.;  Chai, R.;  Liu, D.;  Hu, S.;  Li, M.;  Li, D.;  Wang, H.; Wang, Y., An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells. Scientific reports 2017, 7 (1), 2320.

3. Mu, Z.;  Zhang, S.;  He, C.;  Hou, H.;  Liu, D.;  Hu, N.; Xu, H., Expression of SoxC Transcription Factors during Zebrafish Retinal and Optic Nerve Regeneration. Neuroscience bulletin 2017, 33 (1), 53-61.

4. Lv, F.;  Zhu, C.;  Yan, X.;  Wang, X.; Liu, D., Generation of a mef2aa:EGFP transgenic zebrafish line that expresses EGFP in muscle cells. Fish physiology and biochemistry 2017, 43 (1), 287-294. (通讯作者)

5. Jin, D.;  Zhu, D.;  Fang, Y.;  Chen, Y.;  Yu, G.;  Pan, W.;  Liu, D.;  Li, F.; Zhong, T. P., Vegfa signaling regulates diverse artery/vein formation in vertebrate vasculatures. Journal of genetics and genomics 2017, 44 (10), 483-492.

6. He, Z.;  Guo, L.;  Shu, Y.;  Fang, Q.;  Zhou, H.;  Liu, Y.;  Liu, D.;  Lu, L.;  Zhang, X.;  Ding, X.;  Liu, D.;  Tang, M.;  Kong, W.;  Sha, S.;  Li, H.;  Gao, X.; Chai, R., Autophagy protects auditory hair cells against neomycin-induced damage. Autophagy 2017, 1-21.

7. He, Y.;  Lu, X.;  Qian, F.;  Liu, D.;  Chai, R.; Li, H., Insm1a Is Required for Zebrafish Posterior Lateral Line Development. Frontiers in molecular neuroscience 2017, 10, 241.

8. Gong, J.;  Wang, X.;  Zhu, C.;  Dong, X.;  Zhang, Q.;  Wang, X.;  Duan, X.;  Qian, F.;  Shi, Y.;  Gao, Y.;  Zhao, Q.;  Chai, R.; Liu, D., Insm1a Regulates Motor Neuron Development in Zebrafish. Frontiers in molecular neuroscience 2017, 10, 274. (通讯作者)

2016年

1. Zhang, S.;  Mu, Z.;  He, C.;  Zhou, M.;  Liu, D.;  Zhao, X. F.;  Goldman, D.; Xu, H., Antiviral Drug Ganciclovir Is a Potent Inhibitor of the Proliferation of Muller Glia-Derived Progenitors During Zebrafish Retinal Regeneration. Investigative ophthalmology & visual science 2016, 57 (4), 1991-2000.

2. Wang, X.;  Yuan, W.;  Wang, X.;  Qi, J.;  Qin, Y.;  Shi, Y.;  Zhang, J.;  Gong, J.;  Dong, Z.;  Liu, X.;  Sun, C.;  Chai, R.;  Le Noble, F.; Liu, D., The somite-secreted factor Maeg promotes zebrafish embryonic angiogenesis. Oncotarget2016, 7 (47), 77749-77763. (通讯作者)

3. Shi, Y. W.;  Yuan, W.;  Wang, X.;  Gong, J.;  Zhu, S. X.;  Chai, L. L.;  Qi, J. L.;  Qin, Y. Y.;  Gao, Y.;  Zhou, Y. L.;  Fan, X. L.;  Ji, C. Y.;  Wu, J. Y.;  Wang, Z. W.; Liu, D., Combretastatin A-4 efficiently inhibits angiogenesis and induces neuronal apoptosis in zebrafish. Scientific reports 2016, 6, 30189. (通讯作者)

4. He, Z.;  Zhang, S.;  Song, Q.;  Li, W.;  Liu, D.;  Li, H.;  Tang, M.; Chai, R., The structural development of primary cultured hippocampal neurons on a graphene substrate. Colloids and surfaces. B, Biointerfaces 2016, 146, 442-51. 

2013-2015年

1. Wang, X.;  Wang, X.;  Yuan, W.;  Chai, R.; Liu, D., Egfl6 is involved in zebrafish notochord development. Fish physiology and biochemistry 2015, 41 (4), 961-9. (通讯作者)

2. Wang, X.;  Chong, M.;  Wang, X.;  Wang, H.;  Zhang, J.;  Xu, H.;  Zhang, J.; Liu, D., Block the function of nonmuscle myosin II by blebbistatin induces zebrafish embryo cardia bifida. In vitro cellular & developmental biology. Animal 2015, 51 (3), 211-7. (通讯作者)

3. Xu, M.;  Liu, D.;  Dong, Z.;  Wang, X.;  Wang, X.;  Liu, Y.;  Baas, P. W.; Liu, M., Kinesin-12 influences axonal growth during zebrafish neural development. Cytoskeleton 2014, 71 (10), 555-63. (通讯作者)

4. Wang, X.;  Li, L.; Liu, D., Expression analysis of integrin beta1 isoforms during zebrafish embryonic development. Gene expression patterns : GEP 2014, 16 (2), 86-92. (通讯作者)

5. Sang, Q.;  Zhang, J.;  Feng, R.;  Wang, X.;  Li, Q.;  Zhao, X.;  Xing, Q.;  Chen, W.;  Du, J.;  Sun, S.;  Chai, R.;  Liu, D.;  Jin, L.;  He, L.;  Li, H.; Wang, L., Ildr1b is essential for semicircular canal development, migration of the posterior lateral line primordium and hearing ability in zebrafish: implications for a role in the recessive hearing impairment DFNB42. Human molecular genetics 2014, 23 (23), 6201-11.

6. Li, S.;  Liu, Q.;  Wang, Y.;  Gu, Y.;  Liu, D.;  Wang, C.;  Ding, G.;  Chen, J.;  Liu, J.; Gu, X., Differential gene expression profiling and biological process analysis in proximal nerve segments after sciatic nerve transection. PLoS One 2013, 8 (2), e57000.

7. Jiang, Q.;  Lagos-Quintana, M.;  Liu, D.;  Shi, Y.;  Helker, C.;  Herzog, W.; le Noble, F., miR-30a regulates endothelial tip cell formation and arteriolar branching. Hypertension 2013, 62 (3), 592-8.

8. Huang, Y.;  Wang, X.;  Xu, M.;  Liu, M.; Liu, D., Nonmuscle myosin II-B (myh10) expression analysis during zebrafish embryonic development. Gene expression patterns : GEP 2013, 13 (7), 265-70. (通讯作者) 

2007-2011年

1. Liu, D.;  Krueger, J.; Le Noble, F., The role of blood flow and microRNAs in blood vessel development. Int J Dev Biol 2011, 55 (4-5), 419-29.

2. Krueger, J.;  Liu, D.;  Scholz, K.;  Zimmer, A.;  Shi, Y.;  Klein, C.;  Siekmann, A.;  Schulte-Merker, S.;  Cudmore, M.;  Ahmed, A.; le Noble, F., Flt1 acts as a negative regulator of tip cell formation and branching morphogenesis in the zebrafish embryo. Development 2011, 138 (10), 2111-20.

3. Klein, C.;  Mikutta, J.;  Krueger, J.;  Scholz, K.;  Brinkmann, J.;  Liu, D.;  Veerkamp, J.;  Siegel, D.;  Abdelilah-Seyfried, S.; le Noble, F., Neuron navigator 3a regulates liver organogenesis during zebrafish embryogenesis. Development 2011, 138 (10), 1935-45.

4. Jiang, Q.;  Liu, D.;  Sun, S.;  Hu, J.;  Tan, L.;  Wang, Y.;  Gui, Y.;  Yu, M.; Song, H., Critical role of connexin43 in zebrafish late primitive and definitive hematopoiesis. Fish physiology and biochemistry 2010, 36 (4), 945-51. (共同一作)

5. Jiang, Q.;  Liu, D.;  Gong, Y.;  Wang, Y.;  Sun, S.;  Gui, Y.; Song, H., yap is required for the development of brain, eyes, and neural crest in zebrafish. Biochem Biophys Res Commun 2009, 384 (1), 114-9. (共同一作)

6. Liu, D.;  Wang, Y. X.;  Hu, J. Y.;  Sun, S. N.; Song, H. Y., The effects of connexin43 down regulation on the development of the embryonic heart and vasculature in zebrafish. Progress in Biochemistry and Biophysics 2008, 35 (7), 766-771.

7. Wang, Y. X.;  Qian, L. X.;  Liu, D.;  Yao, L. L.;  Jiang, Q.;  Yu, Z.;  Gui, Y. H.;  Zhong, T. P.; Song, H. Y., Bone morphogenetic protein-2 acts upstream of myocyte-specific enhancer factor 2a to control embryonic cardiac contractility. Cardiovascular research 2007, 74 (2), 290-303.

8. Sun, S. N.;  Gui, Y. H.;  Wang, Y. X.;  Qian, L. X.;  Jiang, Q.;  Liu, D.; Song, H. Y., Effect of dihydrofolate reductase gene knock-down on the expression of heart and neural crest derivatives expressed transcript 2 in zebrafish cardiac development. Chin Med J (Engl) 2007, 120 (13), 1166-71.

九、 获得专利

1、国家发明专利，一种下调真核基因表达的方法及其试剂盒，专利号：ZL201610945971.2

2、国家发明专利，一种特异性用于治疗眼睛血管新生药物的制备方法，专利号：ZL202110737699.X

3、国家发明专利，一种突变型GABRG2转基因斑马鱼癫痫模型的构建方法及应用，专利号：ZL201910396728.3

4、国家发明专利，HPR1基因突变体及其在制备耳聋诊断试剂中的应用，专利号：ZL201910127815.9

5、国家发明专利，遗传性耳聋诱导基因的突变筛选方法及突变检测试剂盒，专利号：ZL202010834744. 9

6、国家发明专利，一种常染色体显性Dnajc17基因突变体及其应用和诊断试剂盒、诊断基因芯片，专利号： ZL201911164469.8

7、国家发明专利，神经节苷脂GM1在用于听力损伤的药物中的用途，专利号： ZL202211207867.5

8、澳大利亚发明专利, Construction method for mutant GABRG2 transgenic zebrafish epilepsy model and applications, Patent number: 2019445928; Name of inventor(s): ZHANG, Qi; LIU, Dong; SHEN, Dingding; CHEN, Juan and KE, Shu'an

十、教学经历

2012-2013-1学期：医学遗传学（本科生），36课时

2012-2013-2学期：细胞生物学技术（研究生），36课时

2012-2013-1学期：高级分子生物学（研究生），42课时

2013年10月：IBRO Schools，Adult zebrafish spinal cord transection

2013-2014-2学期：分子细胞生物学（研究生），12课时

2013-2014-2学期：细胞生物学技术（研究生），36课时

2013-2014-2学期：细胞生物学（本科生），36课时

2014-2015-1学期：高级分子生物学（研究生），36课时

2014-2015-2学期：分子细胞生物学（研究生），12课时

2015-2016-1学期：高级分子生物学（研究生），36课时

2015-2016-2学期：分子细胞生物学（研究生），12课时

2016-2017-1学期：高级分子生物学（研究生），36课时

2016-2017-2学期：分子细胞生物学（研究生），12课时

2016-2017-2学期：分子生物学（本科生），6课时

2019-2020-2学期：合成生物学（本科生），4课时

2020-2021-1学期：医学遗传学（本科生），36课时

2020-2021-2学期：基因工程（本科生），4课时

2020-2021-2学期：高级细胞生物学（研究生），36课时

2020-2021-2学期：组织器官的发育与疾病（研究生），36课时

2021-2022-1学期：医学遗传学（本科生），36课时

2021-2022-2学期：组织器官的发育与疾病（研究生），36课时

2022-2023-1学期：生物化学（本科生），36课时

2022-2023-2学期：组织器官的发育与疾病（研究生），36课时

2023年12月：张謇学院

十一、受邀学术报告和学术交流：

2012年，中科院神经科学研究所，杜久林研究员邀请，报告题目：Identifying MicroRNAs Involved in Zebrafish Embryonic Blood Vessel Development

2013年，中国第三届斑马鱼大会，报告题目：The somite-secreted factor MAEG regulates zebrafish embryonic angiogenesis

2014年，复旦大学附属眼耳鼻喉医院，李华伟教授邀请，报告题目：Angiogenesis & angiogenesis in regeneration

2015年，复旦大学生命科学学院，王红艳教授邀请，报告题目：Angiogenesis & angiogenesis in regeneration

2016年，东南大学生命科学研究院，潘玉峰教授邀请，报告题目：fabp11a gene knockdown caused eye developmental defects in zebrafish

2017年9月，南京医科大学，李琦教授邀请，报告题目：Identification of novel factors involved auditory function using the zebrafish model

2018年6月，东南大学生命科学研究院，柴人杰教授邀请，报告题目：Identification of novel factors involved auditory function

2019年6月，兰州大学附属第二医院，Zebrafish : Science Gateway

2019年8月，山东大学附属山东省耳鼻喉医院，王海波教授邀请，报告题目：Identification of novel factors involved auditory function using the zebrafish model

2020年7月，苏州大学附属第一医院，胡士军教授，报告题目：Identification of novel factors in angiogenesis

2021年7月 ，山东大学生命科学学院，徐志刚教授邀请

2022年8月， 深圳湾实验室 闫致强教授邀请

2022年6月，南京大学附属鼓楼医院 高夏教授邀请

2023年6月，苏州大学附属第一医院 沈振亚教授邀请  

2023年8月，中山大学第五附属医院  何欢欢教授邀请

2023年5月，复旦大学附属眼耳鼻喉医院 舒易来教授邀请

2023年10月，上海交通大学附属第九人民医院 吴昊 教授邀请