植物信号实时监测与地方种质资源保护研究团队

时间:2024-10-09浏览:9139

一、团队简介:

植物电信号和化学信号实时监测及其作用机制研究以番茄和拟南芥为研究对象,结合微流控技术,研究植物在受到环境或外界刺激后,植物体内电信号和化学信号的产生、传输及其互作机制。植物电信号是植物对外界环境刺激做出的最初响应,在植物细胞内、细胞间进行信息传递,进一步启动植物体内的化学信号如过氧化氢、植物激素分子等,从而对植物生长发育、抗病抗逆反应进行调节。该研究方向具有较强的学科交叉性和跨学科特点,涉及植物学、植物生理学、分子生物学、电分析化学、纳米材料和信息等学科,研究成果具有重要理论和现实意义,具有良好的发展前景。

二、团队负责人简介:

孙利军,男,浙江大学植物病理学博士,教授,硕士生导师,先后入选江苏省高校青蓝工程优秀青年骨干教师(2016.05)、江苏省第十四批六大人才高峰高层次人才培养对象(2017.10)以及江苏省高校青蓝工程中青年学术带头人(2021.06)。指导本科生完成国家大学生创新训练和江苏省大学生创新训练各1项、指导指导2014级本科生毕业论文获江苏省2018届本科优秀学位团队论文1项;先后主持江苏省自然科学基金青年项目、国家自然科学基金青年项目、国家自然科学基金面上以及南通市自然科学基金重点项目各1项。研究成果:发表SCI论文34篇(以第一作者或通讯作者发表SCI论文22篇,其中1SCI论文6篇,2SCI论文11篇),H指数为15,引用次数为760次。教育部学位与研究生教育发展中心第五轮学科评估专家,Lab on a Chip等一区TOP国际学术期刊的受邀论文评审人。

三、团队成员:(包括:教师、研究生、本科生等)

1、教师

赵祥强,男,农学博士,副教授,硕士生导师,主要从事植物种质资源学、农田景观生态学、生态农业技术和盐碱地生态改良等方面的研究和科技推广工作。

张华丽,女,哈尔滨工业大学工学博士、副教授、硕士生导师。南通市江海英才、江苏省双创计划人才。主要从事微流控技术、微纳加工等方面的研究工作。主持项目:江苏省自然科学基金项目1项,南通市科技计划项目2项。研究成果:发表学术论文三十余篇,其中SCI收录5篇。申请发明专利11件,其中获授权2.

徐松芝,女,中国科学院植物研究所,植物学博士,讲师,发表SCI论文10篇,主持南通市级项目2项。

汤玲娟,女,华东师范大学分析化学专业硕士,,助理研究员,参与国家自然科学基金面上1项。研究成果:发表 SCI 论文9篇(其中以第一作者发表SCI论文6篇)。发表SCi 论文6篇。

谈蓉,女,湖北大学植物生物学硕士,讲师,发表SCi论文3篇。

周蓉,女,南通大学植物生物学硕士,实验师,发表SCi 论文5篇。

2、研究生:

2022级:刘薇

2023级:赵宇婷,闫续

2024级:田一然,王瑞璇

3、本科生:

2021级:耿心柳、孙雅芳、崔文静、戴颖、陈艺苗、常文彬、黄一芮、崔亚荣,王璇

2022级:赵欣月、陈欣如、庄镕诚、徐亮、钟大计、翟佳羽、段俊琪、陈汇阳、冯绍琳

2023级:马林兵、李梦婷、邰作豪、蒋舒雯、蒋雨阳、宁舒静、彭雪琪、韦伊婷

4、已毕业研究生:

2018级:潘钰、刘莉莉

2019级:孙玲、霍逗逗

2020级:李道东、孙张华、耿迁迁

2021级:张芝瑶、张双玉、李颖

5、已毕业本科生升学情况:

2014级:潘金琳(中国科学院苏州生物医学工程技术研究所)、曹静丽(复旦大学)、王煜垟(复旦大学)、夏志毅(中南大学)、李珍珍(中南大学)、梁云云(南通大学)

2016级:赵丹阳(上海师范大学)、黄文平(苏州大学)、惠美琦(南通大学)、明梦茹(南通大学)

2018级:韩笑(中国药科大学)

2020级:汤熠辉(中国科学院分子植物科学卓越创新中心)、汤玉洁(华东师范大学)、杨影(华东师范大学)、解学谦(云南大学)、田一然(南通大学)

四、研究方向:

1、植物电信号和化学信号实时监测

2、植物电信号和化学信号在植物体内的作用机制

3、微流控系统在植物细胞及根系发育中的应用

4、地方种质资源保护

五、课题:

1、江苏省自然科学青年基金项目:BK20130389 ,植物抗病相关激素的电化学原位检测及其在番茄NAC转录因子抗病功能分析中的应用,2013/07-2016/0615万,已结题,负责人:孙利军。

2、国家自然科学基金青年项目:31400315,植物抗病相关激素的多位点实时检测及其互作研究, 2015/01-2017/1224万,已结题,负责人:孙利军。

3、国家自然科学基金面上项目: 31770399,基于微流控系统及多位点检测的植物抗病相关激素互作研究, 2018/01- 2021/12,直接费用 58万,已结题,负责人:孙利军。

4、南通市自然科学基金重点项目:MS220210382021/07 – 2023/0720万,已结题,负责人:孙利军。

5、耐盐碱观赏稻种质创新及生态应用,纵向10万元,负责人:赵祥强。

6、启东市现代农业产业示范园农业科普教育基地建设,横向44万元,负责人:赵祥强。

7、南通都市农业公园景观农田建设,横向32万元,负责人:赵祥强。

8、江苏省自然科学基金项目:BK20150406,基于集成光流控平台的单细胞间通讯机制研究,2015-2018, 已结题, 负责人:张华丽

9、南通市科技计划项目,MS2016015,应用于单细胞钙瞬变信号分析的光流控芯片系统,2016-2018,已结题,负责人:张华丽

10、南通市科技局项目:JC2018070,南通市外来入侵植物DNA条形码鉴定体系构建,2018/06-2020/063万,已结题,负责人:徐松芝。

11、南通南通市科技局项目:番茄NAC转录因子在细菌性叶斑病抗性中的功能研究,2022/06-2024/063万,在研,负责人:徐松芝。

六、主要论文:

2024

1.Wei Liu#, Zhiyao Zhang#, Xinliu Geng#, Rong Tan, Songzhi Xu, Lijun Sun*, Electrochemical sensors for plant signaling molecules, Biosensors and Bioelectronics, 2025, 116757.IF2023:10.7,中科院1区)

2.Lingjuan Tang#, Zhiyao Zhang#, Ling Sun, Xu Gao, Xinyue Zhao, Xinru Chen, Xingyu Zhu, Aixue Li,* Lijun Sun*, In Vivo Detection of Abscisic Acid in Tomato Leaves Based on a Disposable Stainless Steel Electrochemical Immunosensor, Journal of Agricultural and Food Chemistry, 2024, 72, 31, 1766617674.IF2023:5.7,中科院1区)

3.Lingjuan Tang#, Daodong Li#, Wei Liu#, Yihui Tang, Rongcheng Zhang, Yiran Tian , Rong Tan , Xiaolong Yang*, Lijun Sun*, Microneedle electrochemical sensor based on disposable stainless-steel wire for real-time analysis of indole-3-acetic acid and salicylic acid in tomato leaves infected by Pst DC3000 in situ. Analytica Chimica Acta, 2024, 342875.IF2023:5.7,中科院2区)

2023

1. YangYou,BinLuo, ChengWang,HongtuDong, XiaodongWang,PeichenHou,LijunSun*, AixueLi*,An ultrasensitive probe-free electrochemical immunosensor for gibberellins employing polydopamine-antibody nanoparticles modified electrode, Bioelectrochemistry, 2023, 108331. (IF2023:4.8,中科院2)

2. Lingjuan Tang#, Daodong Li#, Wei Liu , Yafang Sun, Ying Dai, Wenjing Cui, Xinliu Geng, Dayong Li, Fengming Song, Lijun Sun*, Continuous In Vivo Monitoring of Indole-3-Acetic Acid and Salicylic Acid in Tomato Leaf Veins Based on an Electrochemical Microsensor, Biosensors, 2023, 1002. (IF2023:4.84,中科院3)

2022

1. Doudou Huo# , Daodong Li# , Songzhi Xu# , Yujie Tang , Xueqian Xie , Dayong Li , Fengming Song ,Yali Zhang , Aixue Li, Lijun Sun*, Disposable Stainless-Steel Wire-Based Electrochemical Microsensor for In Vivo Continuous Monitoring of Hydrogen Peroxide in Vein of Tomato Leaf. Biosensors 2022, 12, 35. (IF2023:4.84,中科院3)

2. Ling Sun#, Songzhi Xu#, Yihui Tang#, Yuhang Zhou, Meng Wang, Yiran Tian, Guangxi Li, Xinyu Zhu*, Ning Bao*, Lijun Sun*,Disposable stainless steel working electrodes for sensitive and simultaneous detection of indole-3-acetic acid and salicylic acid in Arabidopsis thaliana leaves under biotic stresses. Analytical and Bioanalytical Chemistry, 2022. (IF2023:3.8,中科院2)

3. Songzhi Xu #, Zhiyao Zhang#, Jiajing Zhou, Xiao Han , Kun Song, Haiying Gu, Suqin Zhu*, Lijun Sun*, Comprehensive Analysis of NAC Genes Reveals Differential Expression Patterns in Response to Pst DC3000 and Their Overlapping Expression Pattern during PTI and ETI in Tomato, Genes, 2022, 13, 2015. (IF2023:2.8,中科院3)

4. Songzhi Xu#, Zhanghua Sun#, Lili Liu, Ying Yang, Shuangyu Zhang, Ying Li, Ning Bao, Yali Zhang *, Lijun Sun*, The Cell Wall Regeneration of Tobacco Protoplasts Based on Microfluidic System, Processes, 2022, 10, 2507. (IF2023:2.8,中科院4)

5. Huali Zhang#, Qianqian Geng#, Zhanghua Sun#, Xiaoxiang Zhong, Ying Yang, Shuangyu Zhang , Ying Li, Yali Zhang*, Lijun Sun*, A Dual-Channel Microfluidic Chip for Single Tobacco Protoplast Isolation and Dynamic Capture, Micromachines, 2022, 13, 2109. (IF2023:3,中科院3)

6.Wu Liu, Yuyun Zhang, Xing Fang, Sorrel Tran, Ning Zhai, Zhengfei Yang, Fu Guo, Lyuqin Chen, Jie Yu, Madalene S. Ison, Teng Zhang, Lijun Sun, Hongwu Bian, Yijing Zhang, Li Yang, Lin Xu. Transcriptional landscapes of de novo root regeneration from detached Arabidopsis leaves revealed by time-lapse and single-cell RNA sequencing analyses. Plant communications. 2022, 3, 100306. (IF2023:9.4,中科院1)

2021

1. Ling Sun#, Zhengfei Yang#, Hao Li#, Xiran Lan, Yishun Tang, Wu Liu, Xinyu Zhu*, Ning Bao*, Lijun Sun*, Rapid mapping of the IAA in leaves of Arabidopsis thaliana using a simple paper-based electroanalytical device coupled with microsampling. RSC Adv. 2021, 11, 30392–30397. (IF 2023: 4.036, 中科院3)

2. Lijun Sun#*, Lili Liu#,Xiangyun Lin#, Zhiyi Xia, Jingli Cao, ShaofuXu, Haiying Gu, Haibing Yang, Ning Bao*, Microfluidic Devices for Monitoring the Root Morphology of Arabidopsis Thaliana in situ. Analytical Sciences. 2021, 37(4):605-611. (IF 2023: 1.8, 中科院4)

2020

1. Lijun Sun*, Yu Pan, Jin Wu, Danyang Zhao, Meiqi Hui, Suqin Zhu, Xinyu Zhu, Dayong Li, Fengming Song, Cankui Zhang, Paper-Based Analytical Devices for the Rapid and Direct Electrochemical Detection of Hydrogen Peroxide in Tomato Leaves Inoculated with Botrytis cinerea. Sensors (Basel). 2020, 20(19): E5512.(IF2023:4.84, 中科院3)

2. Kecai He, Heru Wang, Haibing Yang, Lijun Sun*, Wu Liu*, Ning Bao*, Digitalized pencil trace modified electrodes for real time evaluation of salicylic acid in detached Arabidopsis thaliana leaves during regeneration, Analytica Chimica Acta. 2020, 1120:59-66.IF2023:5.7, 中科院2区)

3. Qiong Wang, Xiaohui Li, Lingjuan Tang, Yuejun Fei, Yu Pan, Lijun Sun*,Paper-based electroanalytical devices for in situ determination of free 3-indoleacetic acid and salicylic acid in living Pyropia haitanensis thallus under various environmental stresses, Journal of Applied Phycology. 2020, 32 :485-497.IF2023:2.86, 中科院3区)

4. Jinglun Zhao#, Suqing Zhu#, Lingjuan Tang# , Hao Huang , Mengru Ming, Wenping Huang, Haiying Gu*, Lijun Sun*, Electrochemical behaviour of chloroplast, thylakoid, and PSII on the chitosan modified glassy carbon electrode, Int. J. Electrochem. Sci. 15 (2020) 10499–10508. (IF2023:1.541, 中科院4)

5.Binbin Ye, Guandong Shang, Yu Pan, Zhougeng Xu, Chuanmiao Zhou, Yingbo Mao, Ning Bao, Lijun Sun, Tongda Xu, JiaWei Wang. AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration. Plant cell. 2020, 32:226-241. (IF2023:10, 中科院1)

2019年之前

1. Heru Wang, Xiao-Mei Bi, Zi-Jun Fang, Haibing Yang, Hai-Ying Gu, Lijun Sun*, Ning Bao*, Real time sensing of salicylic acid in infected tomato leaves using carbon tape electrodes modified with handed pencil trace, Sensors & Actuators: B. Chemical. 2019, 286: 104–110. IF2023:8, 中科院1区)

2. Guifang Zhang#, Fei Zhao#, Lyuqin Chen#, Yu Pan, Lijun Sun, Ning Bao, Teng Zhang,  Chun-Xiao Cui, Zaozao Qiu, Yijing Zhang, Li Yang, Lin Xu*, Jasmonate-mediated wound signaling promotes plant regeneration. Nature Plants 2019, 491-497.IF2023:15.8, 中科院1区)

3. Jing Pan#, Fei Zhao#, Guifang Zhang#, Yu Pan, Lijun Sun, Ning Bao, Peng Qin, Lyuqin Chen, Jie Yu*, Yijing Zhang*, Lin Xu*, Control of De Novo Root Regeneration Efficiency by Developmental Status of Arabidopsis Leaf Explants, Journal of Genetics and Genomics. 2019,46:133-140.IF2023:6.6, 中科院2区)

4. Lijun Sun#, Jiajing Zhou#, Jinlin Pan#, Yunyun Liang, Zijun Fang, Yuan Xie, Haibing Yang, Haiying Gu*, Ning Bao*, Electrochemical mapping of indole-3-acetic acid and salicylic acid in whole pea seedlings under normal conditions and salinity, Sensors & Actuators: B. Chemical. 2018, 276: 545-551.IF2023:8, 中科院1区)

5. Lijun Sun# , Yuan xie#, Yongfeng Yan#, Haibing Yang, Haiying Gu *, Ning Bao * Paper-based analytical devices for direct electrochemical detection of free IAA and SA in plant samples with the weight of several milligrams, Sensor Actuat. B Chem. 2017, 247: 336-342. IF2023:8, 中科院1区)

6. Lijun Sun # Xiaojun Liu # Qiaoling Sun Meng Cai Jiajing Zhou Zenglin Dong Ning Bao Haiying Gu* Hong He*. Layer-by-layer assembly of hemoglobin and gold nanoparticles for enhancing the ability of oxygen carrying, Applied Surface Science. 2016, 363,566–571.IF2023:6.3, 中科院2区)

7. Lijun Sun #, Yannan Lu #, Zhongqin Pan #, Tingting, Xiaojun Liu, Ning Bao, Chunmei Yu, Hong He *, Haiying Gu *. Layer-by-layer assembly of hemoglobin-coated microspheres for enhancing the capacity of oxygen carrying, RSC Advances. 2016,6, 59984-59987.IF2023:4.03, 中科院3区)

8. Lijun Sun #, Xiaojun Liu #, Ling Gao, Yannan Lu, Yubin Li, Zhongqin Pan, Ning Bao *, Haiying Gu *. Simultaneous Electrochemical Determination of Indole-3-Acetic Acid and Salicylic Acid in Pea Roots using a Multiwalled Carbon Nanotube Modified Electrode. Analytical Letters. 2015,48(10):1578-1592.IF2023:1.6, 中科院4区)

9. Lijun Sun, Lei Huang, Yongbo Hong, Huijuan Zhang, Fengming Song, Dayong Li *. Comprehensive analysis suggests overlapping expression of rice ONAC transcription factors in abiotic and biotic stress responses. International Journal of Molecular Sciences. 2015, 16(2): 4306-4326.IF2023:5.6, 中科院2区)

10. Lijun Sun # , Qiumei Feng # , Yongfeng Yan, Zhongqin Pan, Xiaohui Li, Fengming Song, Haibing Yang, Jingjuan Xu, Ning Bao *, Haiying Gu *, Paper-based electroanalytical devices for in situ determination of salicylic acid in living tomato leaves. Biosensors and Bioelectronics. 2014, 60:154-160.IF2023:10.7, 中科院1区)

11. Lijun Sun, Huijuan Zhang, Dayong Li, Lei Huang, Yongbo Hong, Xinshung Din, Richard S Nelson, Xueping Zhou, Fengming Song*, Functions of rice NAC transcriptional factors, ONAC122 and ONAC131, in defense responses against Magnaporthe grisea. Plant Mol Biol. 2013, 81(1-2):41-56.IF2023:3.9, 中科院3区)

12. Lijun Sun #, Zhongqin Pan #, jing Xie, Xiaojun Liu, Fengting Sun, Fengming Song, Ning Bao, Haiying Gu *. Electrocatalytic activity of salicylic acid on Au@Fe3O4 nanocomposites modified electrode and its detection in tomato leaves infected with Botrytis cinerea. Journal of Electroanalytical Chemistry. 2013,706: 127-132.IF2023:4.1, 中科院3区)

#表示共同一作,*通讯作者

七、指导学生获奖情况:

1、指导2014级生物技术本科生完成国家级大创项目1项。

2、获2018年江苏省普通高校本专科优秀毕业设计(论文)优秀团队论文1项。

3、指导2019级研究生孙玲同学在2020年江苏省植物生理学会第六届青年学术年会,获得优秀论文报告一等奖1项。

4、指导2020级生物科学本科生获2022全国植物生命科学竞赛江苏赛区二等奖一项。

5、指导2020级生物科学本科生获第十八届“挑战杯”全国大学生课外学术科技作品竞赛“黑科技”展示活动江苏省选拨赛“行星级”一项。

八、合作交流

1、与南通大学顾海鹰、鲍宁、吴增强宜树华 教授建立了良好的合作关系。

2、与浙江大学农业生物技术学院 宋凤鸣教授、李大勇副教授建立了良好的合作关系。

3、与上海中国科学院上海生命科学研究院植物生理生态研究所 徐麟、王佳伟毛颖波 研究员建立了良好的合作关系。

4、与南京农业大学 王暄 教授建立了良好的合作关系。

九、联系方式以及其它信息

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